Expression Technologies Inc

Description

Expression Technologies Inc. is dedicated to providing scientific communities with the necessary and sufficient materials and tools for successful recombinant protein expression in E.coli. There are different sections in our web site describing current technologies used in DNA cloning and protein expression. Host cells give protein expression advantages and disadvantages in each host system. Comparison of expression problems in different host systems are described in Problems and hosts . Decisions to express a recombinant protein as full-length, domains, large fusions or small tags are summarized in a table in Domain and fusion . Technologies to overcome difficulties in cDNA cloning and protein expression, that resulted from protein toxicity, are detailed in Toxic protein cloning and expression . Technologies to improve protein yield, solubility, stability, and activity are provided in Protein yield , Protein solubility , Protein stability , and Protein function sections respectively. Growth media are critically important in protein yield, solubility, stability, and activity. Therefore they are listed in a separate section.

Proteins are soluble in their natural environments. A given natural environment provides all materials and tools necessary and sufficient for a particular protein expression. All above strategies are trying to provide all materials and tools needed for a recombinant protein expression. Different proteins require different materials and tools for its production. Therefore different tools are provided in the cell strains as co-expressed proteins. Our special media provide all known trace metals, minerals, and vitamins. In addition, we also include animal serum extracts which may contain some unknown trace metals, minerals and vitamins needed for some proteins. This is why some proteins are more soluble or completely soluble in our special media.

Expression Technologies Inc. (exptec) is a biotech company founded in 1997. We develop, manufacture, and market innovative reagents for biomedical laboratories and industries.

Protein expression using transgenic animals and cell-free systems are not included here. There is no single system meets all needs of protein expression. Multiple systems may Protein expression using transgenic animals and cell-free systems are not included here. There is no single system meets all needs of protein expression. Multiple systems may be chosen for an expression. However it also increases time, labor, and cost significantly.

Our special media can support E.coli growth to a density to OD 600 =30 to 50 in a shake flask container. As a result, up to 20 times more protein or plasmid DNA can be produced in our media than in LB medium. Many proteins are more soluble, stable, and active in our special media.

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