Kapa Biosystems Inc
- 275 Bunker Hill St
- Charlestown, Massachusetts
- 02129-1834
- Phone: 617.872.0414
- Website
Website Links
Description
A second-generation polymerase engineered for extreme fidelity and robustness - 100x fidelity improvement compared to Taq polymerase. KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi DNA Polymerase results in significant improvements to yield, sensitivity, speed, target length, and the ability to amplify difficult templates (e.g GC-rich) - standardize your high fidelity PCRs with a single enzyme. The engineered KAPA HiFi DNA Polymerase plus optimized buffer system offers: KAPA HiFi DNA Polymerase is also available with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling. The KAPA HiFi HotStart DNA Polymerase is really amazing! Our lab routinely performs cloning, vector construction, and DNA sequencing and we have used various high fidelity polymerases from companies like Invitrogen, Promega, Roche, and Stratagene for a long period of time but never obtained such outstanding results as we did with the KAPA HiFi DNA Polymerase from Kapa Biosystems. We were able to easily amplify desired fragments up to 9 kb with very high yields and specificity. Furthermore, KAPA HiFi required no optimization. This product is excellent - now making our high fidelity PCR more reliable and consistent. - Dr. Jeanette Lescano-Balindong, Plant Breeding, Genetics and Biotechnology, International Rice Research Institute, Philippines
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