Lawrence Berkeley National Lab

Description

We are performing inverse PCR on these 2,300 lines to obtain a sequence tag that will precisely localize the insertion when the corresponding genomic region is sequenced. To date we have attempted this protocol on 891 EP lines. We obtained an average of 179 bp of flanking sequence; for 739 lines (83%) we obtained sufficient sequence (>25 bp) to provide a useful sequence tag. The 17% of the lines that failed in this initial procedure will be reanalyzed using a second set of PCR primers that amplify from the other end of the P element and/or by using an additional restriction enzyme.

Products & services

Similar companies nearby