Polyplus Transfection

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Description

Polyplus-transfection ® SA is the exclusive worldwide licensee of U.S. Patent No. 6,013,240, European Patent No. 0770140, and foreign equivalents, which cover transfection compositions having nucleic acids and a cationic polymer based on polyethylenimine (PEI), and use of this cationic polymer in nucleic acid transfection. Polyplus-transfection ® sells branded PEI-based transfection reagents under its exclusive license, including the PEIpro™.

Figure 3. siRNA/INTERFERin®-HTS complexes are stable for up to 4 hours at room temperature. siRNA/INTERFERin®-HTS complexes were formed according to the manufacturer‘s recommendations using 10 nM siRNA duplexes and incubated at room temperature for the indicated time. Subsequently, HeLa cells were transfected in 96-well plates with 10 nM siRNA duplexes targeting lamin A/C following a reverse transfection protocol according to the manufacturer‘s recommendations. Lamin A/C expression was measured 24 hours after transfection by branched DNA assay and normalized to GAPDH expression.

“ In our hands, FectoPRO™ gives the highest protein production yields we ever reached using transient transfection. Our company offers custom services including protein production in mammalian cells. Being able to produce a lot more protein without revisiting our expression system opens a whole new range of possibilities for our antibody and protein production services”.

Polyplus-transfection manufactures and markets proprietary transfection reagents for gene expression, RNA interference, High Throughput Screening and bioproduction.

PEIpro™ is a transfection reagent for the production of recombinant proteins and antibodies in HEK-293 and CHO cells grown in serum-free media. PEIpro™ benefits from the major product improvements required for Transient Gene Expression (TGE) in terms of manufacturing process, product characterization and proven transfection efficiency.

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