Textco Biosoftware

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[If working with the demo version of GCK, saving is disabled, simply continue to step 16 below]. If working with a ‘Trial Version’ or the ‘Full Version’ of the application, choose File > Save As… , name the file my construct#7 , and save it in your own folder – please do not replace the tutorial files provided because others might want to use them later. Since cloning the hsp70 fragment into the BamHI site has an equally likely chance of occurring in either orientation, these constructs ( construct#6 and construct#7 ) represent two possible outcomes in a real experiment. You will be able to use these constructs and GCK to predict gel patterns as detailed in Tutorial 9: Running Gels and Orientation Analysis . This demonstrates how easy it is to replace DNAs and to generate constructs with inserts in either orientation.

You should now see the hsp70 construct with the middle BamHI site missing. The actual nucleotide sequence has been changed as specified in Figure 2.25 with the changed nucleotide being indicated in lower case in the actual sequence. [If working with the demo version of GCK, saving is disabled, simply continue to step 8 below]. If working with a ‘Trial Version’ or the ‘Full Version’ of the application, choose File > Save As… , name the file myhsp70_Bam- , and save it in your own folder – please do not replace the tutorial files provided because others might want to use them later. You will need this file again in Tutorial 10: Making Illustrations.

[If working with the demo version of GCK, saving is disabled, simply continue to step 14 below]. If working with a ‘Trial Version’ or the ‘Full Version’ of the application, choose File > Save As… , name the file myconstruct#6 , and save it in your own folder – please do not replace the tutorial files provided because others might want to use them later.

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